fitc conjugated rat anti cd169 (Bio-Rad)
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Fitc Conjugated Rat Anti Cd169, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd169+fitc/Rat+anti+Mouse+CD169/pmc12748623-235-4-8
Average 95 stars, based on 194 article reviews
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Staining:Article Title: Targeting Toll-Like Receptor 2: Polarization of Porcine Macrophages by a Mycoplasma-Derived Pam2cys Lipopeptide. Article Snippet: MoMΦ were washed, resuspended in 0.1 mL per tube, and stained with Zombie Aqua dye (BioLegend, San Diego, CA, USA), and after 30 min of incubation at room temperature, cells were washed with PBS supplemented with 0.5% bovine serum albumin (BSA). .. Cells were then resuspended in 0.1 mL of PBS, and staining with monoclonal antibodies was performed using mouse anti human CD14-PerCP (Tuk4; Miltenyi Biotec, Bergisch Gladbach, Germany), mouse anti pig MHC class II DR-FITC (2E9/13, Bio-Rad Antibodies, Kidlington, UK), MHC class I (JM1E3, Bio-Rad Antibodies), CD163-PE (2A10/11, Bio-Rad Antibodies), Article Title: LSD1 cooperates with non-canonical NF-κB signaling to regulate marginal zone B cell development Article Snippet: .. Slides were stained with a primary antibody cocktail of Article Title: Targeting Toll-Like Receptor 2: Polarization of Porcine Macrophages by a Mycoplasma-Derived Pam2cys Lipopeptide Article Snippet: MoMΦ were washed, resuspended in 0.1 mL per tube, and stained with Zombie Aqua dye (BioLegend, San Diego, CA, USA), and after 30 min of incubation at room temperature, cells were washed with PBS supplemented with 0.5% bovine serum albumin (BSA). .. Cells were then resuspended in 0.1 mL of PBS, and staining with monoclonal antibodies was performed using mouse anti human CD14-PerCP (Tuk4; Miltenyi Biotec, Bergisch Gladbach, Germany), mouse anti pig MHC class II DR-FITC (2E9/13, Bio-Rad Antibodies, Kidlington, UK), MHC class I (JM1E3, Bio-Rad Antibodies), CD163-PE (2A10/11, Bio-Rad Antibodies), Article Title: Heterogeneity of Phenotypic and Functional Changes to Porcine Monocyte-Derived Macrophages Triggered by Diverse Polarizing Factors In Vitro Article Snippet: Then, the cells were harvested with 10 mM EDTA in PBS and transferred to 5 mL round bottom tubes (Corning, Corning, NY, USA). .. Cells were first stained with Zombie Aqua viability dye (BioLegend, San Diego, CA, USA) (30 min, room temperature), then they were washed with PBS supplemented with 0.5% bovine serum albumin (BSA), and subsequently stained with several murine monoclonal antibodies (mAbs): anti-porcine CD16-PE (clone G7, Thermo Scientific Pierce, Rockford, IL, USA), anti-human CD14-PerCP-Cy5.5 (clone Tuk4, Miltenyi Biotec, Bergisch Gladbach, Germany) [ ], CD163-PE (clone 2A10/11, Bio-Rad Antibodies, Kidlington, UK), Article Title: Cerebrospinal fluid CD4+ T cell infection in humans and macaques during acute HIV-1 and SHIV infection Article Snippet: Cryopreserved PBMC and CSF cell samples were thawed and stained with fluorescent conjugated antibodies specific for lineage and activation markers (BD Biosciences unless otherwise noted). .. Rhesus macaque specimens were stained with the following: CD14-APC (M5E2), CD16-PE-Cy7 (3G8), CD3-AL700 (SP34-2), CD20-PE-CF594 (2H7), CD4-BV605 (OKT4, Biolegend), CD8a-BV785 (RPA-T8), CD95-BV421 (DX2), and CD28-PE-Cy5 (CD28.2); and markers of activation: CD38-PE (OKT10), CD69-BUV395 (FN50), Bioprocessing:Article Title: Targeting Toll-Like Receptor 2: Polarization of Porcine Macrophages by a Mycoplasma-Derived Pam2cys Lipopeptide. Article Snippet: MoMΦ were washed, resuspended in 0.1 mL per tube, and stained with Zombie Aqua dye (BioLegend, San Diego, CA, USA), and after 30 min of incubation at room temperature, cells were washed with PBS supplemented with 0.5% bovine serum albumin (BSA). .. Cells were then resuspended in 0.1 mL of PBS, and staining with monoclonal antibodies was performed using mouse anti human CD14-PerCP (Tuk4; Miltenyi Biotec, Bergisch Gladbach, Germany), mouse anti pig MHC class II DR-FITC (2E9/13, Bio-Rad Antibodies, Kidlington, UK), MHC class I (JM1E3, Bio-Rad Antibodies), CD163-PE (2A10/11, Bio-Rad Antibodies), Article Title: Targeting Toll-Like Receptor 2: Polarization of Porcine Macrophages by a Mycoplasma-Derived Pam2cys Lipopeptide Article Snippet: MoMΦ were washed, resuspended in 0.1 mL per tube, and stained with Zombie Aqua dye (BioLegend, San Diego, CA, USA), and after 30 min of incubation at room temperature, cells were washed with PBS supplemented with 0.5% bovine serum albumin (BSA). .. Cells were then resuspended in 0.1 mL of PBS, and staining with monoclonal antibodies was performed using mouse anti human CD14-PerCP (Tuk4; Miltenyi Biotec, Bergisch Gladbach, Germany), mouse anti pig MHC class II DR-FITC (2E9/13, Bio-Rad Antibodies, Kidlington, UK), MHC class I (JM1E3, Bio-Rad Antibodies), CD163-PE (2A10/11, Bio-Rad Antibodies), Article Title: Heterogeneity of Phenotypic and Functional Changes to Porcine Monocyte-Derived Macrophages Triggered by Diverse Polarizing Factors In Vitro Article Snippet: Then, the cells were harvested with 10 mM EDTA in PBS and transferred to 5 mL round bottom tubes (Corning, Corning, NY, USA). .. Cells were first stained with Zombie Aqua viability dye (BioLegend, San Diego, CA, USA) (30 min, room temperature), then they were washed with PBS supplemented with 0.5% bovine serum albumin (BSA), and subsequently stained with several murine monoclonal antibodies (mAbs): anti-porcine CD16-PE (clone G7, Thermo Scientific Pierce, Rockford, IL, USA), anti-human CD14-PerCP-Cy5.5 (clone Tuk4, Miltenyi Biotec, Bergisch Gladbach, Germany) [ ], CD163-PE (clone 2A10/11, Bio-Rad Antibodies, Kidlington, UK), Immunopeptidomics:Article Title: Targeting Toll-Like Receptor 2: Polarization of Porcine Macrophages by a Mycoplasma-Derived Pam2cys Lipopeptide. Article Snippet: MoMΦ were washed, resuspended in 0.1 mL per tube, and stained with Zombie Aqua dye (BioLegend, San Diego, CA, USA), and after 30 min of incubation at room temperature, cells were washed with PBS supplemented with 0.5% bovine serum albumin (BSA). .. Cells were then resuspended in 0.1 mL of PBS, and staining with monoclonal antibodies was performed using mouse anti human CD14-PerCP (Tuk4; Miltenyi Biotec, Bergisch Gladbach, Germany), mouse anti pig MHC class II DR-FITC (2E9/13, Bio-Rad Antibodies, Kidlington, UK), MHC class I (JM1E3, Bio-Rad Antibodies), CD163-PE (2A10/11, Bio-Rad Antibodies), Article Title: Targeting Toll-Like Receptor 2: Polarization of Porcine Macrophages by a Mycoplasma-Derived Pam2cys Lipopeptide Article Snippet: MoMΦ were washed, resuspended in 0.1 mL per tube, and stained with Zombie Aqua dye (BioLegend, San Diego, CA, USA), and after 30 min of incubation at room temperature, cells were washed with PBS supplemented with 0.5% bovine serum albumin (BSA). .. Cells were then resuspended in 0.1 mL of PBS, and staining with monoclonal antibodies was performed using mouse anti human CD14-PerCP (Tuk4; Miltenyi Biotec, Bergisch Gladbach, Germany), mouse anti pig MHC class II DR-FITC (2E9/13, Bio-Rad Antibodies, Kidlington, UK), MHC class I (JM1E3, Bio-Rad Antibodies), CD163-PE (2A10/11, Bio-Rad Antibodies), Article Title: Heterogeneity of Phenotypic and Functional Changes to Porcine Monocyte-Derived Macrophages Triggered by Diverse Polarizing Factors In Vitro Article Snippet: Then, the cells were harvested with 10 mM EDTA in PBS and transferred to 5 mL round bottom tubes (Corning, Corning, NY, USA). .. Cells were first stained with Zombie Aqua viability dye (BioLegend, San Diego, CA, USA) (30 min, room temperature), then they were washed with PBS supplemented with 0.5% bovine serum albumin (BSA), and subsequently stained with several murine monoclonal antibodies (mAbs): anti-porcine CD16-PE (clone G7, Thermo Scientific Pierce, Rockford, IL, USA), anti-human CD14-PerCP-Cy5.5 (clone Tuk4, Miltenyi Biotec, Bergisch Gladbach, Germany) [ ], CD163-PE (clone 2A10/11, Bio-Rad Antibodies, Kidlington, UK), Blocking Assay:Article Title: LSD1 cooperates with non-canonical NF-κB signaling to regulate marginal zone B cell development Article Snippet: .. Slides were stained with a primary antibody cocktail of other:Article Title: Multi-Antigen Imaging Reveals Inflammatory DC, ADAM17 and Neprilysin as Effectors in Keloid Formation Article Snippet: Recombinase Polymerase Amplification:Article Title: Cerebrospinal fluid CD4+ T cell infection in humans and macaques during acute HIV-1 and SHIV infection Article Snippet: Cryopreserved PBMC and CSF cell samples were thawed and stained with fluorescent conjugated antibodies specific for lineage and activation markers (BD Biosciences unless otherwise noted). .. Rhesus macaque specimens were stained with the following: CD14-APC (M5E2), CD16-PE-Cy7 (3G8), CD3-AL700 (SP34-2), CD20-PE-CF594 (2H7), CD4-BV605 (OKT4, Biolegend), CD8a-BV785 (RPA-T8), CD95-BV421 (DX2), and CD28-PE-Cy5 (CD28.2); and markers of activation: CD38-PE (OKT10), CD69-BUV395 (FN50), Activation Assay:Article Title: Cerebrospinal fluid CD4+ T cell infection in humans and macaques during acute HIV-1 and SHIV infection Article Snippet: Cryopreserved PBMC and CSF cell samples were thawed and stained with fluorescent conjugated antibodies specific for lineage and activation markers (BD Biosciences unless otherwise noted). .. Rhesus macaque specimens were stained with the following: CD14-APC (M5E2), CD16-PE-Cy7 (3G8), CD3-AL700 (SP34-2), CD20-PE-CF594 (2H7), CD4-BV605 (OKT4, Biolegend), CD8a-BV785 (RPA-T8), CD95-BV421 (DX2), and CD28-PE-Cy5 (CD28.2); and markers of activation: CD38-PE (OKT10), CD69-BUV395 (FN50), |


